%0 Journal Article %A Khishvand, Z %A Hosseini, M %A Sharifi, A %A Tabatabaei, SM %A Khosravani, SAM %T Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj %J Armaghane Danesh %V 24 %N 6 %U http://armaghanj.yums.ac.ir/article-1-2100-en.html %R 10.52547/armaghanj.24.6.1116 %D 2019 %K E.coli, ESBL, PCR, CTXM1, %X Background & aim: Resistance to β-lactam antibiotics in the clinical isolates, in most cases is caused by β-lactamase enzymes. In recent years, The incidence of broad-spectrum β-lactamase enzymes (ESBLs) among clinical isolates especially E.coli is greatly increased, since the β-lactamase have several subfamilies, using universal primers designed to detect the following complete families could be useful. β-lactamase producing enzymes (ESBLs) of E. coli has created many problems for patients. β-lactamase CTX-M-1 gene is the cause of resistance. The aim of this study was to evaluate CTX-M-1gene in E.coli. Methods: In this practical study, susceptibility of isolated bacteria to 13 antibiotics were indicated by disk diffusion method according to CLSI guidelines and strains were analyzed for the presence of widespread β-lactamase enzymes via two-disc synergy method. ThusŲŒ the prevalence of CTX-M1 ESBL gene samples were determined using PCR and the data were analyzed using ANOVA. Results: A total of 200 isolates of E.coli were isolated. The presence of CTX-M-1 gene were also isolated using the PCR method. From 200 strains studied, 62 (31%), of strains produced ESBL. After PCR processing of 62 produced ESBL, 43 isolates (69.4%) were identified as CTX-M-1 genes. Also, antibiotic susceptibility test showed the highest percentage of resistance to Cotrimoxazole antibiotic (50%) and the lowest antibiotic resistance to imipenem (0%). Conclusion: The results of this study showed the high percentage of β-lactamase resistance among of E.coli strains. This is a serious public hazard that should be pointed out to measures for preventing this hazard. Considering the sensitivity of the studied beta-lactam resistant isolates and isolates in Iran to imipenem, a carbapenem with a non-beta-lactam antibiotic is recommended for the treatment of nosocomial infections caused by these strains. %> http://armaghanj.yums.ac.ir/article-1-2100-en.pdf %P 1116-1126 %& 1116 %! %9 Research %L A-10-1366-1 %+ Cellular and Molecular Research Center, yasuj University of Medical Sciences, yasuj, Iran %G eng %@ 1728-6506 %[ 2019