TY - JOUR T1 - In Vitro Maturation and Fertilization of Cryopreserved Germinal Vesicle Stage Oocytes in NMRI Mice, Using Ethylene Glycol and DMSO TT - بلوغ و لقاح آزمایشگاهی تخمک ژرمینال وزیکول پس از انجماد شیشه‌ای با استفاده ازضد یخ اتیلن گلیکول و دی‏متیل سولفوکساید در موش JF - yums-armaghan JO - yums-armaghan VL - 16 IS - 4 UR - http://armaghanj.yums.ac.ir/article-1-328-en.html Y1 - 2011 SP - 341 EP - 353 KW - Oocyte KW - Cumulus cells KW - Vitrification KW - Maturation N2 - Background & Aim: Cryopreservation of oocytes is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of exposure to combination of cryoprotectants and vitrification on immature mouse oocytes with or without cumulus cells. Methods: This was an experimental study conducted at Yasouj University of Medical Sciences in 2010. Immature oocytes with and without cumulus cells were isolated from ovaries of mice 4-6 weeks of age. They were vitrified in conventional straw using ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose as vitrification solution or exposed to vitrification solution without subjected to liquid nitrogen. After warming, oocytes were assessed for nuclear maturation and fertilization. The collected data were analyzed with one-way ANOVA and Tukey test. Results: Survival and fertilization rates in vitrified oocytes with cumulus cells were significantly lower than the control group (p M3 ER -