Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice
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M Modaresi 1, M Basravi2 , I Sajadia2 |
1- Department of Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran , mehrdad_modaresi@hotmail.com 2- 2Department of Psychology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran |
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Abstract: (6491 Views) |
Background & aim: Anxiety is a normal feeling which is experienced in threatening situations and can affect neural system. Wide spread of anxiety forces many people to permanent use of anti-stress drugs especially benzodiazepines. This study was carried out to compare the effects of balm extract and diazepam on anxiety adjustment.
Methods: In this experimental study 70 female mice weighing approximately 25 to 30 g were studied in seven treatments groups including control, placebo, anxiety, diazepam, 50, 100, and 200 mg/kg of balm’s hydro-alcoholic extract. Drug and extract doses were injected IP. After receiving the last dose, anxiety was induced using dark box and was evaluated by an elevated plus-maze. In order to increase the activity, the animals were kept in a box with black walls for 5 minutes. Then, in order to evaluate the response of anxiety in elevated plus maze transferred and count the time spent in the open arms for 5 minutes (as an indicator of anxiety) were observed and recorded. Obtained data were analyzed using SPSS 11.5 software.
Results: The results of tests on the treatment showed that the hydro alcoholic extract in 200 mg/kg dose increased the time of presence in open arms significantly which shows anxiety reduction. Also, movement activities of mice were significantly higher in this dose in proportion to diazepam.
Conclusion: According to results, hydro alcoholic extract of balm in 200 mg/kg dose can be a suitable replacement for diazepam in reducing anxiety reflexes.
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Keywords: Melissa officinalis, Anxiety, Diazepam, Mice |
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Full-Text [PDF 194 kb]
(1783 Downloads)
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Type of Study: Research |
Subject:
Physiology Received: 2015/06/29 | Accepted: 2015/12/5 | Published: 2015/12/9
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