Background & Aim: Breast cancer is the most common malignancy in women . Studies have shown that increased in methylation of CpG islands (CpG island hyper methylation, CIHM), is one of the important mechanisms in gene down regulation. DAP-Kinase protein plays an important role in the process of Apoptosis. Interleukin-17 is an proinflammatory cytokine and inflammation,is one of the factors  that affect on gene methylation . the purpose of this study was to evaluate the polymorphism of the IL-17 gene promoter methylation Dap-kinase and its relationship to breast cancer.

Methods: In this case - control study, A total of 40 Women with Breast cancer and 40 healthy women in Iran were examined.DNA was extracted by saluting out method and Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method and To study gene promoter methylation Dap-kinase, MSPCR method was used.data were compared in both groups by using Pearson’s chi-square and Hardy-weinberg equilibrium test.

RESULTS: Results confirm the fact that, there is a relationship between DAP-kinase gene promoter methylation and breast cancer disease So that the promoter of this gene in patients than in healthy individuals was much more methylated( p<0.05) . On the other hand there is no significant coloration between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05)

Conclusion: Due to the fact, that promoter genes methylation is one of the mechanisms of epigenetic genes silencing, it seems that DAP-kinase gene promoter methylation increases is associated with the risk of breast cancer in women.