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Change of Astrocytes Number in CA2 Subfield of Hippocampus after Spatial Learning
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M Jahanshahi   , Y Sadeghi , SA Hosseini , N Naghdi |
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Abstract: (4254 Views) |
| ABSTRACT:
Introduction & Objective: The hippocampal formation is a part of
limbic system that plays an important role in memory and learning.
The hippocampus is divided into three subfields: CA1, CA2 and
CA3. In addition to pyramidal neurons and interneurons, the
astrocytes play important roles in hippocampus, probably in
memory and learning. In fact, learning needs some instruments for
information storage and information maintenance mechanisms like
memory. On the other hand, memory is always accompanied with
learning. This study was conducted to evaluate the changes in
number of astrocytes in CA2 subfield of hippocampus in rats due to
spatial learning.
Materials & Methods: In this experimental study, with the use of
Morris Water Maze and two other techniques (Reference and
Working memory methods) in the behavioral laboratory of
physiology department of Pasteur institute, 15 male Wistar rats
were used. Five rats were included in control group and 10 rats in
the other two groups (each 5 rat). After performing the educational
experiments, animal brain was removed and after histological
processing, the slides were stained with PTAH to show the
astrocytes. The obtained data were analyzed by SPSS, using T
test and variance analysis.
Results: The results showed a significant difference in astrocytes
number of CA2 area between control and reference memory
group. Also the difference between control and working memory
groups was significant. Comparing the two educated groups, a
significant difference was found in the number of astrocytes in
those groups.
Conclusion: The number of astrocytes in CA2 area of
hippocampus of rats increased due to spatial learning. |
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| Keywords: Hippocampus, CA2, Astrocytes, Spatial Learning, PTAH staining |
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Full-Text [PDF 399 kb]
(1092 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/15 | Accepted: 2015/06/15 | Published: 2015/06/15
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