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Evaluation of Antinociceptive Effect of Galbanum by Formalin Test in Mice
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A Zarifkar   , M Karami-Kheirabad 1, M Edjtehadi , K Rastgar , M Ghalje |
| 1- , marya.karami@gmail.com |
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Abstract: (5546 Views) |
| ABSTRACT:
Introduction & Objective: Ferula gummosa Boiss (Apiaceae) is a
perennial plant native to Iran and Afghanistan. Like many plants,
this species contains active biological compounds including
monoterpenes, alfa pinene and alkaloids which have been
demonstrated in phytochemical analysis of different extract of this
species. In Iranian traditional medicine Ferula gummosa has been
used in intestinal disorders, asthma and convulsion. Previous
studies showed that Galbanum is effective in relieving pain in hot
plate test model. In this study analgesic effect of intra peritoneal
injection of Galbanum (Ferula exudates) was evaluated by formalin
test and was compared with the analgesic effect of morphine and
non-steroidal anti-inflammatory drugs.
Material & Methods: This experimental study was done from July
2003 through 2004 in Shiraz University of Medical Sciences. 120
male Syrian mice weighing 25-32g, in ten groups were used in this
study. Mice were pretreated intra peritoneally (i.p.) 15 minutes
before formalin injection, with Galbanum (100 mg/kg), morphine
(4&6 mg/kg) and sodium salicylate (100&200 mg/kg). In vehicle
groups, equal volume of solvent (normal saline and/or DMSO) was
injected 15 minutes before formalin test. In diclofenac- sodium
(5&10 mg/kg, i.p.) and piroxicam (50 mg/kg, i.p.) groups, animals
were pretreated with these drugs 30 minutes before formalin
injection. Control group was not pretreated with any drug before
formalin test. Data obtained from formalin test were expressed as
Mean±SEM and were analyzed by ANOVA and Tukey test.
Results: Results of the present study showed that intra peritoneal
injection of Galbanum (100 mg/kg) reduced the pain score in acute
phase of formalin test (2.28±0.05) and also in chronic phase
(1.09±0.03). These differences were significant, in comparison with
control values, in both phases (p<0.05).
Conclusion: According to our findings, intra peritoneal injection of
galbanum (100 mg/kg), like NSAIDs and morphine, reduces acute
and chronic pain in formalin test. Moreover, Galbanum along with
diclofenac has additive effect at chronic phase of formalin test,
which means that it may have an anti-inflammatory effect as well. |
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Full-Text [PDF 158 kb]
(1287 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/15 | Accepted: 2015/06/15 | Published: 2015/06/15
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