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Introduction of a Simple and Rapid Modified blood Agar Media for Mass Cultivation of Leishmania Spp
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P Habibi   , MH Motazdian 1, F Esfandiari , M Fakhar |
| 1- , motazedm@sums.ac.ir |
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Abstract: (4371 Views) |
| Introduction & Objective: Identification of Lieshmania is based not only on external characteristics, but also on some internal characteristics including molecular structure of the organism. It is necessary to perform these tests with 1010 organisms. In vitro mass cultivation of Leishmania needs different rich liquid culture media with FCS (Fetal Calf Serum) at 22-26°. Thus, introduction of cheap media which can guarantee the growth of parasites seems necessary. This study aimed at introducing of a cheap, simple, and modified blood agar media for mass cultivation of Lesihmania.
Materials & Methods: In this experimental study, at first the growth rate of of Leishmania major, Leishmania infantum and Leishmania tropica, on blood agar and BHI (Brian Heart Infusion) broth with 20% FCS were compared. Then the schisodeme pattern of RFLP (Restriction Fragment Length Polymorphism) for Leishmania major cultivated in two cultures was compared. Finally, 40 samples taken from patients suspicious of cutaneous leishmaniasis, referred to Shiraz university in 1380-1381, were cultured in the new introduced media to determine its usefulness for mass cultivation of Leishmania Spp.
Results: The promastigote growing ratio in blood agar media and BHI broth for Leishmania major, Leishmania infantum and Leishmania tropica were 5.1, 9.4, and 5.7, respectively after one week for L. major and L.infantum and two weeks for L. tropica. Similarity of leishmania major RFLP schisodeme pattern cultivated on both media revealed unchanged natural characters of the mass cultivated parasites.Ten samples isolated from patients were mass cultivated on NNN and blood agar successfully and all reached 1010 parasite.
Conclusion: Use of blood agar media for mass cultivation of Leishmania is a cost-effective method which increases the growth rate of parasite and decreases the risk of contamination. |
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| Keywords: Modified blood agar, Leishmania Spp, Growth of Leishmania Parasite |
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Full-Text [PDF 209 kb]
(1663 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/6 | Accepted: 2015/06/6 | Published: 2015/06/6
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