Background and purpose: in recent years, in order to improve power, speed, the increase in the volume of the musculature, preventing sports injuries and maintain the muscle performance athletes use from different resistance exercises and food supplements. In this regard, present study has been conducted with the aim of comparison the influence of an 8 week period consumption of creatine (2 gr.kg-1.day-1 in 1st week and 0.48 gr.kg-1.day-1during 2nd to 8th weeks) and glutamine (1 gr.kg-1.day-1 from first to eighth weeks) along with resistance exercise on level of ALP of female mice. Materials and methods: This experimental study was done on 80 Small adult female mice of Surrey species (28 ± 5 gram). The animals were randomly divided into 8 groups of: resistance exercise, resistance exercise + creatine, resistance exercise + glutamine, resistance exercise + glutamine + creatine, creatine, glutamine, creatine + glutamine and control groups (N= 10). Resistance exercise (5 days a week) was including: climbing (4 sets, 5 times repetition with two minutes rest between the sets) from a ladder (with the height of one meter and including 26 steps) and bearing 30 percent of the weight of the Mouse body (hanging from tail) in the first week and the increasing it up to 200 percent of body weight till the last week of the experiment. During 48 hours after the last practice session of resistance exercise, the blood sample was taken and the the level of ALP has been measured. Findings:The results showed that the level of ALP enzyme in creatine + glutamine + resistance exercise groug had been increased in comparison with the control group (144.3 ± 15.86 in comparison with 234.7 ± 25.69 U.L-1 P < 0.05). Conclusion: The results of this research indicate Creatine and Glutamine supplementation consumption along with resistance exercise increases in the level of ALP enzyme in the liver of mice.