[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing & Abstracting
DOAJ
GOOGLE SCHOLAR
..
:: Volume 20, Issue 4 (7-2015) ::
__Armaghane Danesh__ 2015, 20(4): 333-345 Back to browse issues page
Effects of prenatal stress on seizure threshold and development of hippocampus in mice offspring
M Kahali1 , Sh Oryan1 , H Sadraee2 , Gh Kaka , S Parsaee3 , H Mohseni kouchesfehani3
1- 1Department of Physiology, Kharazmi University, Tehran, Iran
2- 2Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- 3Department of Developmental Biology, Kharazmi University, Tehran, IranCenter, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (4858 Views)
Background & aim: The effect of stress on the nervous system activity may be due to alterations in the structure of the nervous system. In this study, the effects of maternal stress was examined on the seizure threshold and hippocampal structure of their offspring during pregnancy. Methods: In this study, thirty pregnant mice were divided into two groups. 1) No stress group and 2) Stress group that received one hour immobilization stress from 1 to 14 day of pregnancy. The seizure threshold test was performed in offspring by injection of Pantilen tetrazol (PTZ). To study of the hippocampus development, the mouse offspring were divided into three groups: The control group no received immobilization stress on pregnant rat and their offspring also no received PTZ. The Sham group no received immobilization stress on pregnant rat but their offspring received PTZ. and experimental group that pregnant rat received immobilization stress and their offspring received PTZ. At the end of the experiences, all offsprings were killed by chloroform and their hippocampus fixed in and processed and 5µm sections were prepared and stained by the H&E method. The pyramidal and granular layers thickness in the Hippocampus were measured using Motic software and the number of cells in these layers and the number of blood vessels in the molecular and polymorphic layers were counted. The data were analyzed using ANOVA and T test. Results: The results showed a significant increase in seizure threshold in offspring whose mothers were under stress, compared with offspring whose mothers were not under stress. Average thickness of hippocampal pyramidal and granular in the experimental group than in the control group showed a significant increase (p<0.001). in addition, mean thickness of pyramidal and granular layers of hippocampus significantly increased in the experimental group compared to control group. The number of cells in hippocampal and granular layers significantly decreased in the experimental group when compared with control and sham groups (p<0.001). Conclusion: Prenatal stress can cause an increase in seizure threshold and also impair offspring's development and their hippocampal structure.
Keywords: hippocampus, pregnancy, PTZ, stress, seizure threshold
Full-Text [PDF 1216 kb]   (1338 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/01/28 | Accepted: 2015/06/21 | Published: 2015/07/23
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

kahali M, oryan S, sadraee H, kaka G, parsaee S, mohseni kouchesfehani H. Effects of prenatal stress on seizure threshold and development of hippocampus in mice offspring . armaghanj 2015; 20 (4) :333-345
URL: http://armaghanj.yums.ac.ir/article-1-38-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 4 (7-2015) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4645