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Effect of 5- Azacytidine on the Cell Growth Inhibition and Apoptosis Induction Lung Cancer A549 Cell Line
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M Sanaei Jahromi1    , F Kavousi 2 |
1- Non-Communicable Diseases Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
2- Non-Communicable Diseases Research Center, Jahrom University of Medical Sciences, Jahrom, Iran , kavoosifraidoon@gmail.com |
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Abstract: (2105 Views) |
Background & aim: Lung cancer is one of the most important causes of cancer death. Epigenetic changes include; DNA methylation, histone distillation, and microRNA can lead to the silencing of cancer-suppressing genes, resulting in cancer. The aim of this study was to determine the effect of 5-azacitidine on lung cancer.
Methods: The present laboratory study was conducted in 2019. 300,000 cells were selected for each experimental group. Lung cancer cells of class A549 were treated with 5-azacitidine and the cell viability; apoptotic cells and gene expression were evaluated by MTT, flow cytometry and PCR, respectively. Data were analyzed using one-way ANOVA and t-test.
Results: The results indicated that 5-aza cytidine significantly inhibited cell growth, induced apoptosis, decreased expression of methyltransferase DNA genes (DNMT1, DNMT3A and DNMT3B) and increased expression of pKA kinase inhibitors of pK4, pK4-dependent kin of pIN-14.
Conclusion: 5-Azacitidine could increase the expression of tumor-inhibiting genes and induce apoptosis in lung cancer cells by inhibiting the expression of methyltransferase genes. The percentage of apoptotic cells after 24 and 48 hours was 8.89 and 54.7, respectively (p <0.001). The maximum rate of apoptosis was observed after 48 hours.
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| Keywords: 5-AZA-CdR, DNA methyltransferases, Tumor suppressor genes, Lung cancer |
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Full-Text [PDF 1410 kb]
(469 Downloads)
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Type of Study: Research |
Subject:
Immunology
Received: 2020/03/31 | Accepted: 2020/10/4 | Published: 2020/11/7
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