:: Volume 24, Issue 1 (4-2019) ::
__Armaghane Danesh__ 2019, 24(1): 60-71 Back to browse issues page
Investigation of the Protective Effect(s) of Wheat Germ on the Hippocampus of Rats Exposed to Lead
SH Naderi1, H Moravati 2, F Sasani3
1- Department of Veterinary Medicine, University of Tehran, Tehran, Iran
2- Department of Veterinary Medicine, University of Tehran, Tehran, Iran , hmorovvati@ut.ac.ir
3- Department of Pathology, University of Tehran, Tehran, Iran
Abstract:   (216 Views)
Background & aim: Due to the toxic effects of lead at low levels which causes several disorders, such as weakness, impaired ability and behavioral thinking and children's learning, loss of hearing and vision, damage to cerebellum gray cells and brain hippocampus. The purpose of this study was to evaluate the protective role of wheat germ in controlling oxidative stress and tissue damage of lead-exposed brain hippocampus.
Methods: In the present experimental study, 30 female Wistar rats (240 ± 20 g) were divided into 6 groups: control (without treatment), treat with 10 mg lead, wheat germ extract with 100 and 200 mg, synchronous lead and wheat germ extracted at doses of 100 and 200 mg. On the 36th day, the mice were killed and their brain were dissected. After the stabilization of the samples, the dewatering, clarifying, and molding processes took place. The molds were cut in a thickness of 5-7 micrometers and stained with H & E. The collected data was analyzed by one-way ANOVA and Tukey's test.
Results: A significant differences was observed in the treatment group regarding the number of pyramidal cells in CA1, the number of granulary granulomatous cells in the teeth and the thickness of the vaginal layers of the teeth compared to the control group.On the other hand, at concentration of 20 mg lead group, the 20 mg lead group with 100 mg of wheat germ, the number of pyramidal cells in CA1 and the number of cells and the thickness of the granular ventricular layer of the teeth were significantly different compared to the other groups.  No significant difference was observed in the thickness of the teething tubes, the number of pyramidal cells in CA1 and granular layer cells, and the weight of the mice in all groups at dose of 20 mg lead and gram wheat germ wheat extract 100 and 200 mg compared to the control howerer, the weight of the brain in all groups was significantly higher than the control group.
Conclusion: According to the findings of the present study, the use of wheat germ extracts due to the oxidative anti stress effect could be effective in improving the lesions induced by lead on the rat's hippocampus. Wheat germ extract had the ability to compensate for lead-induced lesions in the hippocampus, which depends on the dose administered.
Keywords: Wheat germ, lead, gingival extracts, hippocampus, rats
Full-Text [PDF 413 kb]   (7 Downloads)    
Type of Study: Research | Subject: Anatomy
Received: 2019/03/6 | Accepted: 2019/03/6 | Published: 2019/03/6

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Volume 24, Issue 1 (4-2019) Back to browse issues page