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:: Volume 23, Issue 6 (1-2019) ::
__Armaghane Danesh__ 2019, 23(6): 737-746 Back to browse issues page
Identification of Blastocystis sp. subtypes from human using 18s rRNA in Northwest of Iran
Jalal Mohamadi Mr1 , Jamal Hallaj zadeh Dr2 , Mehrdad Rostami Mr3 , Saber Raeghi Dr 4, Hadi Mirahmadi Dr5 , Fares Bahrami Dr6 , Reza Shafiei Dr7 , Arezoo Bozorgomid Dr8
1- Department of Health affairs, Shiraz University of Medical Sciences, Shiraz, Iran
2- Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
3- Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran
4- Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran , saberraeghi@gmail.com
5- Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
6- Zoonoses research center, Kurdistan University of Medical Sciences, Sanandaj, Iran
7- Department of Pathobiology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
8- Asadabad School of Medical Sciences, Asadabad, Iran.
Abstract:   (379 Views)
Background and Aim: Blastocystis sp. is an anaerobic parasite that lives in the digestive system of humans with a vast variety of non-human hosts as the zoonotic parasite. This study was carried out to identify subtypes and to investigate the genetic variation of Blastocystis sp. from human samples in Urmia, Tabriz & Maragheh cities Northwest of Iran.
Methods: In this descriptive-cross sectional study, 300 human stools from Jan till Oct 2017 were randomly selected from treatment centers in North West of Iran. Based on microscopic observation, positive samples examined by DNA barcoding methods for detection of Blastocystis sp. subtypes and their sequences were analyzed. 
Results: Out of 300 samples, 248(82.7%) cases were male and 52(17.3) were female. Of these, 20 samples diagnosed Blastocystis sp. positive with the microscopic method. 16 of them were examined by molecular method (PCR) and sequences analyses showed that 3 subtypes (STs) containing ST1, ST2, and ST3.
Conclusion: There is a zoonotic cycle of this parasite in this studied area. By subtypes identifying of Blastocystis sp. in different hosts with distributed prevalence, it is possible to prevent the disease and complications from it and stop the transmission cycle.
Keywords: Blastocystis sp., subtypes, 18s rRNA, Northwest, Iran
Full-Text [PDF 286 kb]   (11 Downloads)    
Type of Study: Research | Subject: Parasitology
Received: 2018/06/25 | Accepted: 2018/11/3 | Published: 2018/12/25
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mohamadi J, hallaj zadeh J, rostami M, raeghi S, mirahmadi H, bahrami F, et al . Identification of Blastocystis sp. subtypes from human using 18s rRNA in Northwest of Iran. Armaghane danesh. 2019; 23 (6) :737-746
URL: http://armaghanj.yums.ac.ir/article-1-2232-en.html


Volume 23, Issue 6 (1-2019) Back to browse issues page
ارمغان دانش Armaghane danesh
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