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Comparison of Antibiotic Resistance and Frequency of Virulence Genes in Urinary and Fecal Escherichia coli in Patients with Urinary Tract Infection
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M Bahadori1    , M Motamedifar2 , AA Derakhshandeh 3, A Motamedi Borujeni1 , M Ali Nejad4 , Z Nazari1 |
1- Department of Pathobiology, Shiraz University, Shiraz, Iran,
2- Shiraz HIV / AIDS Research Center, Health Foundation, Shiraz University of Medical Sciences, Shiraz, Iran,
3- Department of Pathobiology, Shiraz University, Shiraz, Iran, , drkhshnd77@gmail.com
4- Department of Urology, Kerman University of Medical Sciences, Kerman, Iran |
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Abstract: (3501 Views) |
Abstract
Background & Aim: Urinary tract infections are one of the most important bacterial infections, and the most common cause of these infections is E.coli. Considering the importance of E.coli in the development of urinary tract infection in the gastrointestinal tract, the present study was conducted to compare the E.coli causative agent of E.coli infection with E.coli dominating the natural flora of the gastrointestinal tract on the basis of antibiotic resistance and some acute genes.
Methods: For this descriptive study, 30 women with urinary tract and urinary tract infections were collected from the 30 patients. After colonization and confirmation, three colonies were randomly selected from different parts of each plate (fecal samples and each individual sample) were selected for review. The frequency of hly D and cnf1 genes were determined by PCR method and a phenotypic study of antibiotic resistance of isolates were performed using antibiotic disks. Data were analyzed using descriptive statistics and Chi-square test.
Results: Generally 90 isolates of urine and 90 fecal isolates were isolated from the samples. Comparison of the results of urine and fecal isolates showed that the prevalence of examined genes in urine strains was higher than that of fecal isolates (p <0.05). The highest resistance to stool and urinary flora was observed with trimethoprim sulfamethoxazole and gentamicin antibiotics. The highest susceptibility to ciprofloxacin antibiotic (32.2%) was found in urine isolates. In uric acid and fecal Escherichia coli, 37.7% and 46.6% of isolates were resistant to three antibiotics and more. In 9 out of 30 patients, antibiotic resistance and acute genes had the same pattern.
Conclusion: Most fecal flora is not an infectious agent, but isolates with higher genetic parameters are effective in causing infection. Considering the greater resistance to trimethoprim sulfamethoxazole and gentamicin antibiotics in both groups, fecal flora may be involved in transferring the gene to antibiotic resistance to urine.
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| Keywords: Escherichia coli Causing Urinary Tract Infection, Normal E.coli, Antibiotic |
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Full-Text [PDF 233 kb]
(807 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2017/11/19 | Accepted: 2018/07/4 | Published: 2018/08/3
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