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:: Volume 23, Issue 2 (6-2018) ::
__Armaghane Danesh__ 2018, 23(2): 134-145 Back to browse issues page
Study of the effect of hyaluronic acid on the behavior of skin fibroblasts cultured on chitosan scaffold
Seyedeh-Sara Hashemi Dr1 , Seyedeh-Somayeh Rajabi MSc2 , Reza Mahmoudi Professor3 , Amir Ghanbari Dr.4 , Mehrzad Jafari Barmak Dr 5
1- Assistant professor Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2- Msc Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Professor Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Assistant professor Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
5- Assistant professor Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran , mehrzadj14@gmail.com
Abstract:   (390 Views)
Introduction:
Tissue engineering is a new method for replacing tissue fragments degraded by biodegradable polymers, which is provided as a three-dimensional scaffold for the growth and propagation of stem cells. In this study, chitosan was used to evaluate the migration and proliferation of fibroblasts in the presence of hyaluronic acid.
Material and Method:
In this study, scaffold chitosan was prepared for the growth of fibroblastic cells. Then, for further studies, scaffolds were divided into the following groups:
Group 1: Chitosan scaffold with hyaluronic acid
Group 2: Chitosan without hyaluronic acid scaffold
Group 3 (control 1): Hyaluronic acid fibroblast cell
Control group 2: Hyaluronic acid-free fibroblastic cell
The human foreskin was prepared and the fibroblast cells in the dermal layer were centrifuged after separation, and the cells were transferred to the culture flasks with DMEM medium and stored in a CO2-encoded incubator. After several passage cells, 10,000 cells were transferred to 96 wells containing DMEM medium and MTT and DAPI staining were investigated and the fibroblasts were infiltrated on the chitosan scaffold. The obtained results were analyzed by SPSS software, ANOVA and post hoc Tukey test.
Results:
The results showed that the mean survival rate of chitosan without hyaluronic acid scaffold in 24 hours was significantly higher than hyaluronic acid and chitosan groups with hyaluronic acid (P <0.05). The mean survival in the control group without hyaluronic acid was significantly higher in 48 hours compared with the chitosan scaffold with and without hyaluronic acid and the control group with hyaluronic acid (P <0.05). The average survival rate of chitosan scaffold with and without hyaluronic acid in 72 hours compared to control groups with and without hyaluronic acid was not statistically significant.
 Conclusion: The results of this study showed that chitosan scaffolds with regard to hydrophilicity, better biocompatibility with fibroblast cells, suggest that chitosan may be synthesized in structures that are synthesized to repair affected areas of skin. Have a proper and complementary role.
Keywords: Chitosan, scaffold, fibroblasts, hyaluronic acid, skin
Full-Text [PDF 680 kb]   (46 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/09/29 | Accepted: 2018/04/17 | Published: 2018/05/22
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Hashemi S, Rajabi S, Mahmoudi R, Ghanbari A, Jafari Barmak M. Study of the effect of hyaluronic acid on the behavior of skin fibroblasts cultured on chitosan scaffold. Armaghane danesh. 2018; 23 (2) :134-145
URL: http://armaghanj.yums.ac.ir/article-1-1951-en.html


Volume 23, Issue 2 (6-2018) Back to browse issues page
ارمغان دانش Armaghane danesh
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