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:: Volume 23, Issue 2 (6-2018) ::
__Armaghane Danesh__ 2018, 23(2): 146-159 Back to browse issues page
The effect of Green Tea hydroalcohalic extract of Camellia sinensis on AQP1 Gene expression in Proximal tubuls of Gentamicin treated rat kidney
Saideh Parvardeh
, Saideh.Parvardeh@yahoo.com
Abstract:   (237 Views)
Background and aim: Gentamicin is an aminoglycoside antibiotic used to treat a variety of bacterial infections particularly Gram-negative ones. However, the nephrotoxicity and oxidative damage caused by gentamicin limit its long-term clinical use. Grean tea contains some phenol compounds called catechins which have antioxidant and anti-inflammatory effects.  AQP1 is a protein that make a water channel in renal tubules that transported water during the appical and baso-lateral of the renal tubuls. To cheching the protective effect of Grean tea extract(GTE) in AQP1 gene expression in Gentamicin treated rat kidney, this search has prepared.
Methods:In this experimental study,30 Wistar male rats 6-4 weeks 160 - 200g were used and divided into 5 groups (n=6) to receive saline; gentamicin 20 mg/kg ; GTE 200 mg/kg/B.W. ; GM+ GTE 200 mg/kg/B.W. ;intraperitoneal for 10 days and control group. Biochemical changes in blood urea and creatinine levels evaluated   Based on the reaction of Bertolt and Chlorometric and Gaffa method and renal changes were evaluated by means of  histochemistry and immunohistochemistry. The Data was analyzed with using of one-way ANOVA, followed by Tukey's HSD tests using SPSS 21.
Results:  The mean of Levels of creatinine and blood urea increased significantly in the First  group compared to the control  group   (P<0.05)  and The mean of Levels of creatinine and blood urea increased   in the third  group compared to the control  group(P<0.05). Other  investigation demonstrated that necrosis and inflammation of Kidney and accumulation of eosinophilic hyaline materials in the some of  renal tubules and accumulation of mononuclear inflammatory cells and reduction in the expression of AQP1 in proximal tubules caused by gentamicin could not improve by use of green tea extract concentration 200 mg / kg.
Conclusion: From the findings of this study, it can be concluded that green tea extract in this dose and duration can not have a protective effect against the renal toxicity of gentamicin
Keywords: Keywords: AQP1, Creatinine, Gentamicin, Grean tea extract, Histopathology Nephrotoxicity, urea.
Full-Text [PDF 1147 kb]   (25 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/07/16 | Accepted: 2018/05/14 | Published: 2018/05/22
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Parvardeh S. The effect of Green Tea hydroalcohalic extract of Camellia sinensis on AQP1 Gene expression in Proximal tubuls of Gentamicin treated rat kidney . Armaghane danesh. 2018; 23 (2) :146-159
URL: http://armaghanj.yums.ac.ir/article-1-1876-en.html


Volume 23, Issue 2 (6-2018) Back to browse issues page
ارمغان دانش Armaghane danesh
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