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Relationship Between MicroRNA129-2 Expression and Cervical Cancer in Patients Infected with HPV
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ZH Nazeri Givi1   , Kh Onsory 2, E Moslemi3 |
1- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran,
2- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran, , onsory@gmail.com
3- Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran |
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Abstract: (4403 Views) |
Abstract
Background and Aim: Cervical cancer is the fourth most common cancer that is the first cause of mortality among women in the world and the seventh rank among all cancers. One of the risk factors for cervical cancer is infection with the papilloma virus. On the other hand, microRNAs have been suggested as new markers for cervical cancer diagnosis. Meanwhile, microRNA129-2 is a cellular proliferation inhibitor and an invertebrate cellular agent. The aim of this study was to evaluate the expression of microRNA129-2 in women with papilloma virus infected cervical cancer and compare it with women with cervical cancer without infection with papilloma virus and healthy group.
Methods: In this semi-experimental study, 20 paraffin tissue samples from women with papilloma virus infected cervical cancer, 20 samples of paraffin tissue from women with cervical cancer without infection of the papilloma virus and 20 samples of normal pap smear from Mirzakochek Khan Hospital The forest was collected in Tehran in 1394. After de-paraffinization, extraction of RNA was performed and expression of miR129-2 was investigated using Real Time PCR method among the groups. Data was analyzed using Grafpad prism6 software.
Results: According to the results a significant decrease was seen in the expression of this gene in infected patients compared to control group (p = 0.0004). Also, expression of miR129-2 in infected tissues of papilloma viruses was reduced in comparison with non-contaminated cancerous tissues (p = 0.0001). Although this decrease in expression was observed between the patients without infection with the papilloma virus and the control group, it was not statistically significant (p = 0.083). There was no significant relationship between expression of miR129-2 with age (p = 0.99) and grade of disease (p = 0.39).
Conclusion: Considering the reduced expression of miR129-2 in cancerous samples, the expression of miR129-2 expression can be considered as a valuable initial diagnostic agent and it plays an important role in determining the prognosis of cervical cancer.
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| Keywords: Cervical cancer, Human papilloma virus, miR129-2 expression |
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Full-Text [PDF 937 kb]
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Type of Study: Research |
Subject:
Special
Received: 2017/02/10 | Accepted: 2018/01/7 | Published: 2018/01/22
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