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Investigation of Liraglutide Effects on Changes of Uterine Tissue, Estradiol and FSH Hormones in NMRI Mice
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S Atashnezhad1   , N Hayati Roudbari 2, S Mohammadi Gorji , K Parivar1 |
1- 1Department of Biology, Azad University Science and Research Branch, Tehran, Iran
2- 1Department of Biology, Azad University Science and Research Branch, Tehran, Iran , hayati@srbiau.ac.ir |
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Abstract: (5994 Views) |
Abstract
Background & aim: Liraglutide has used for treatment of obesity and type 2 diabetes, which are main factors of infertility. Regarding that, uterus as the most important organ reproductive system is under vulnerable, particularly by chemical drugs, the purpose of this research was to investigated the effects of Liraglutide on uterine tissue changes and Estradiol and FSH hormones in NMRI mice in In vivo condition has been investigated.
Methods: This experimental study was performed on 28 adult NMRI female rats (8 to 12 weeks old) weighing between 25 and 30 grams. Mice were randomly divided into four groups (seven) including control, sham and two experimental groups. The control group was no injected and the sham and experimental groups 1 and 2 respectively received a solvent (distilled water) and liraglothedi at doses of 0.6 mg / kg and 1.8 mg / kg respectively. The injections were subcutaneously administered daily and in one hour. After 30 days of injection, the mice were deeply anesthetized by the ether and blood was removed from heart. After staining, samples were examined by optical microscopy. The collected data were analyzed using one-way ANOVA and Tukey's test.
Results: The weight of the mice in the experimental groups 1 and 2 significantly (p <0.01) and (p <0.01) compared with control and sham groups has decreased. Also, the thickness of the uterus (from the lumen to the last layer) and the diameter of the lumen showed a significant decrease (p <0.05) and (p <0.01) in the experimental groups. A significantly decreased was seen on endometrial thickness in the experimental groups 1 and 2 (p <0.01) and (p <0.001). Also, the thickness of primer in the experimental group also decreased significantly (p <0.05) in comparison with the control group. In addition, using the drug the number of uterine glands in experimental groups 1 and 2 showed a significant increase (p <0.05) and (p <0.01), and their diameter increased in both groups. Also, estradiol and FSH levels were decreased and increased, respectively.
Conclusion: Treatment with liraglutadiene is associated with weight loss and tissue thickness of the uterus. Due to the disturbance in the hormonal system, which is probably due to the specificity of the drug, it will also be limited due to the reduction in estradiol, which is a female hormone.
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| Keywords: Liraglutide, uterine changes, estradiol hormone |
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Full-Text [PDF 547 kb]
(1270 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2017/01/15 | Accepted: 2017/07/23 | Published: 2017/07/25
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