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The Effect of Bone Marrow Mesenchymal Stem Cell Culture in the Treatment of Azoospermic Infertility induced by Busulfan Balb / C mice
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A Mozafar1   , D Mehranani 2, A Vahdati1 , SE Hosseini1 , M Forouzanfar3 |
1- Department of Biology, Islamic Azad University, Fars, Iran
2- Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , mehrabad@sums.ac.ir
3- Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran |
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Abstract: (5684 Views) |
Abstract
Background & aim: Prescribing chemotherapy drugs in patients with cancer can be the mainstay of infertility. Therefore, the aim of this study was to investigate The Effect of Bone Marrow Mesenchymal Stem Cell Culture in the Treatment of Azoospermic Infertility induced by Busulfan Balb / C mice
Abstract
Background & aim: Prescribing chemotherapy drugs in patients with cancer can be the mainstay of infertility. Therefore, the aim of this study was to investigate The Effect of Bone Marrow Mesenchymal Stem Cell Culture in the Treatment of Azoospermic Infertility induced by Busulfan Balb / C mice
Methods: In this experimental study, 18 mice of Balb-C race were randomly divided into control, azoospermic and secreta groups. The animals in the control group did not receive any drug treatment. To stop spermatogenesis and induction of azospermide, a dose of busulfan (10 mg / kg body weight) was injected to azoospermic and cyclic rats at intervals of 21 days. 35 days after the last injection of busulfan, the medium used for the culture of mesenchymal stem cells of the bone marrow was injected into the testis of the rat secreta group. Eight weeks after inoculation, the testicles of all animals were separated and histologically and histomorphologically examined. Data were analyzed by one-way ANOVA.
Results: Histologic findings showed that in the azoospermic group, the spermatogenic cells were significantly reduced, and the vacuoles were observed in the germinal epithelium. After treatment with secreta, the epithelium and spermatogenesis were observed. Also, histomorphometric findings showed that cell thickness and area, total diameter, cross-sectional area and spermatogenesis index of seminal tubes were increased in the secreta group compared to the azoospermic group (p <0.001), but decreased compared to the control group (P <0.05). Also, the number and density of seminal tube in control group and secreta decreased compared to Azospermic group (p <0.001). There was no significant difference between lumen diameter and area of menises in all three groups (p>0.05).
Conclusion: The media used in the cultivation of bone marrow mesenchymal stem cells (Secreta) can cause spermatogenesis back in busulfan-induced azoospermic mice. These findings can be attributed to previous findings in male infertility treatment.
Methods: In this experimental study, 18 mice of Balb-C race were randomly divided into control, azoospermic and secreta groups. The animals in the control group did not receive any drug treatment. To stop spermatogenesis and induction of azospermide, a dose of busulfan (10 mg / kg body weight) was injected to azoospermic and cyclic rats at intervals of 21 days. 35 days after the last injection of busulfan, the medium used for the culture of mesenchymal stem cells of the bone marrow was injected into the testis of the rat secreta group. Eight weeks after inoculation, the testicles of all animals were separated and histologically and histomorphologically examined. Data were analyzed by one-way ANOVA.
Results: Histologic findings showed that in the azoospermic group, the spermatogenic cells were significantly reduced, and the vacuoles were observed in the germinal epithelium. After treatment with secreta, the epithelium and spermatogenesis were observed. Also, histomorphometric findings showed that cell thickness and area, total diameter, cross-sectional area and spermatogenesis index of seminal tubes were increased in the secreta group compared to the azoospermic group (p <0.001), but decreased compared to the control group (P <0.05). Also, the number and density of seminal tube in control group and secreta decreased compared to Azospermic group (p <0.001). There was no significant difference between lumen diameter and area of menises in all three groups (p>0.05).
Conclusion: The media used in the cultivation of bone marrow mesenchymal stem cells (Secreta) can cause spermatogenesis back in busulfan-induced azoospermic mice. These findings can be attributed to previous findings in male infertility treatment.
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| Keywords: Mesenchymal Stem Cells, Secreta, Busulfan, Azoospermia |
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Full-Text [PDF 280 kb]
(1473 Downloads)
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Type of Study: Research |
Subject:
Physiology
Received: 2016/12/26 | Accepted: 2017/06/30 | Published: 2017/07/25
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