[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 22, Number 3 (6-2017) ::
__Armaghane Danesh__ 2017, 22(3): 295-310 Back to browse issues page
The Effect of Conditioned Media from Bone Marrow-Derived Stem Cells in Treatment of Azoospermic infertility Induced by Busulfan in Bulb–C Mice
Mr ahmad mozafar1, Dr davood mehrabani 2, Dr Akbar Vahdati3, Dr Ebrahim Hosseini4, Dr Mohsen Forouzanfar5
1- Ph.D. student 1 Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran, 2 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran,
2- Assistant Professor 3 Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, , mehrabad@sums.ac.ir
3- Professor 1 Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran, 2 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran,
4- Associate Professor 1 Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran, 2 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran,
5- Assistant Professor 4 Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
Abstract:   (328 Views)

Background & aim: Administration of chemotherapy in cancer patients can cause infertility. This study was conducted to evaluate the effect of conditioned media from bone marrow-derived stem cells (secreta) in treatment of azoospermic infertility induced by Busulfan in Bulb–C mice.

Methods: In this experimental study,eighteen Balb-C mice were randomly divided into three groups of control,azoospermia and secreta. The control group did not receive any treatment. In order to stop endogenous spermatogenesis and induce azoospermia, two doses of 10 mg/kg of busulfan with 21 days interval were injected. In secreta group, 35 days after the last injection of busulfan, conditioned media from bone marrow-derived stem cells was injected into the testes. After 8 weeks, testes were removed for histological and histomrphometric evaluations. the collected data were statistically analyzed using one-way analysis of variance.

Results: Histological findings showed, in azoospermia group, spermatogenic cells had dramatically decreased and vacuolated spaces in the germinal epithelium were observed. After treatment with secreta, the germinal epithelium layer and spermatogenesis process significantly improved. The histomrphometric findings indicated that cellular diameter, cellular area, total diameter, cross-sectional area and spermatogenesis index in the seminiferous tubules in the conditioned media were more than azoospermia group (p<0.001), but less than control group (p<0.05). The number of tubules and numerical density of the tubules in control and conditioned media groups were less than azoospermic group (p<0.001). The diameter and luminal area in all groups did not have any significant difference (p>0.05).

Conclusion: Injected conditioned media from bone marrow-derived stem cells (secreta) into testis could induce spermatogenesis in seminiferous tubules of azoospermic mice. That these findings can be added to the previous literature for treatment of male infertility. 

Keywords: Mesenchymal stem cells, secreta, Busulfan, Azoospermia
Full-Text [PDF 280 kb]   (62 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2016/12/26 | Accepted: 2017/06/30 | Published: 2017/07/25
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

mozafar A, mehrabani D, Vahdati A, Hosseini E, Forouzanfar M. The Effect of Conditioned Media from Bone Marrow-Derived Stem Cells in Treatment of Azoospermic infertility Induced by Busulfan in Bulb–C Mice . Armaghane danesh. 2017; 22 (3) :295-310
URL: http://armaghanj.yums.ac.ir/article-1-1647-en.html
Volume 22, Number 3 (6-2017) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.074 seconds with 797 queries by yektaweb 3461