:: Volume 22, Issue 5 (12-2017) ::
__Armaghane Danesh__ 2017, 22(5): 608-622 Back to browse issues page
Effect of High Intensity Endurance Training on p53 and Cytochrome-c Gene Expression in Male Rat Soleus Muscle
F Seyedgomi 1, J Bashiri 2, F Gholami 3
1- Department of Physical Education, Tabriz Branch, Islamic Azad University, Tabriz, Iran,
2- Department of Physical Education, Tabriz Branch, Islamic Azad University, Tabriz, Iran, , bashiri.jabbar@gmail.com
3- Department of Physical Education and Sports Sciences, Shahrood University of Technology, Shahrood, Iran.
Abstract:   (2167 Views)
Abstract
Background and aim: Recent research has suggested that exercise training is potential to influence the process of programmed cell death. However, little is known about the effect of high intensity endurance exercise on apoptotic factors. Therefore, in the present study the effect of high intensity endurance training on p53 and cytochrome-c gene expression in male rats' Soleus muscle was investigated.
 
Methods: In this experimental study sixteen 3-month-old male rats were randomly divided into two groups of endurance training (n=8) and control (n=8). Exercise program included three months endurance training at the intensity of 75-80% of VO2max with the frequency of 5 days per week. Forty-eight hours following the last training session, the soleus muscle of the animals were extracted and p53 and cytochrome-c gene expression were assessed using Real Time-PCR method. Data were analyzed by independent t-test at the significance level of P<0.05.
 
Results: No significant difference was seen between the experimental and control groups regarding p53 gene expression (P=0.67). However, cytochrome-c gene expression was significantly lower in the experimental group than the control group (P=0.001).
 
Conclusion: High intensity endurance training decreased cytochrome-c gene expression which is likely mediated by improved mitochondrial membrane stability and decreased permeability.  It may influence the process of apoptosis in soleus muscle. However, it needs to be examined and validated in future studies.
 
 
Keywords: High intensity endurance training, p53 gene, Cytochrome-c gene, Soleus muscle
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Type of Study: Research | Subject: Sport Physiology
Received: 2016/11/8 | Accepted: 2017/09/19 | Published: 2017/12/4


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Volume 22, Issue 5 (12-2017) Back to browse issues page