Abstract

Background and aim:  Biofilm formation is one of the most notable mechanisms that contributes to antibiotic resistance in P.aeruginosa , The aim of this study was to determine the effects of methanol extrac of Quercus brantii,  Pistacia atlantica, Elaeagnus angustifolia Leaves  on biofilm formation P. aeruginosa strains.

Methods: this study is an experimental study, that extraction with Maceration (powder mixed with 80% methanol) using rotary evaporator method. done. The minimum inhibitory concentration of extracts was determined by broth microdilution. Biofilm formation was investigated using the microtiter plate and stained with crystal violet. Collected data were analyzed using ANOVA and Duncan test.

Results: The minimum inhibitory concentration of Quercus brantii, Pistacia atlantica, Elaeagnus angustifolia extracts against Pseudomonas aeruginosa was 0.625 mg/ml whereas, the Minimum bactericidal concentration of Quercus brantii jaft was 1.25 mg/ml and for Pistacia atlantica and Elaeagnus angustifolia were 2.5 mg/ml. The mean percentage of of Pseudomonas aeruginosa biofilm inhibition by extracts of Quercus brantii (jaft), Pistacia atlantica, Elaeagnus angustifolia were 60.24, 57.35, and 72.63 % respectively.

Conclusion: Methanolic extracts of medicinal plants Quercus brantii, Pistacia atlantica, Elaeagnus angustifolia has anti-bacterial and anti-biofilm effect against Pseudomonas aeruginosa. So that Elaeagnus angustifolia extract had a capability to inhibit biofilm formation and also showed a significant difference compare to Quercus brantii jaft, Pistacia atlantica extract. With further study it can be used of  these extracts as a supplement to inhibit bacterial biofilm.