:: Volume 21, Issue 3 (6-2016) ::
__Armaghane Danesh__ 2016, 21(3): 305-313 Back to browse issues page
Antimicrobial effect of hydroalcoholic extract of saturega multica and zinc oxide namoparticle on coagulase gene expression on clinical and standard samples of MRSA (Methicilin resistant staph aureus)
F Moridikia1, SAM Khosrovani2, M Ghaedi3, M Zoladl4, A Moridikia5, R Mohseni1, AK Alamdari6, A Sharifi2
1- Student Research Committee, Yasuj University of Medical Sciences
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Yasuj, Iran,
3- Institute of Chemistry, Yasuj University, Yasuj, Iran
4- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Yasuj, Iran
5- Chemical Injuries Research Center, Baghiatollah University of Medical Sciences, Tehran, Iran.
6- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Yasuj, Iran,
Abstract:   (5539 Views)

Background & aim: Methicillin-resistant Staphylococcus aureus (MRSA) as nosocomial pathogens have been causing severe and deadly diseases around the world.  Coagulase is an important virulence factor for this bacterium and exisist in all staphylococcus aureus isolates. In recent years, studies carried out into the effects of medicinal plants, nanoparticles against bacteria and pathogenic bacteria’s expression genes. The aim of this study was to investigate the antimicrobial effect of satureja mutica hydroalcoholic extract, zinc oxide nanoparticle, and zinc complex on the coagulase gene expression in clinical and standard isolates of methicillin-resistant staphylococcus aureus (MRSA)

Methods: In the present quasi-experimental study, using micro dilution and MTT, the minimum inhibitory concentration (MIC) of hydro-alcoholic extracts of satureja mutica and zinc oxide nanoparticles were tested against MRSA strains. By polymerase chain reaction ((RT- PCR) coa gene expression in satureja mutica extract and zinc oxide nanoparticles treated were qualitatively evaluated. Data were analyzed using statistical tests

Results: The MIC of hydro alcoholic extract of Satureja mutica  for standard strains and clinical S. aureus  were 3000 and 1500 µg/ml respectively, whereas, the MIC  of nanoparticle zinc oxide on Standards and clinical isolates  were 40 and 20 µg/ml.The hydro alcoholic extract of Satureja mutica on MIC concentration has significant inhibitory effect on coagulase gene expression but no effect was seen for clinical and standard MRSA.

Conclusion: The results show a decline in the coa gene expression in vitro by RT- PCR method using satureja mutica  , but no effect on gene expression Housekeeping arc C. An inhibitory effect was observed on bacterial growth by zinc oxide nanoparticles, but no inhibitory effect on gene expression was seen.

Keywords: MRSA, CuO, Cu complex, coagulase, Satureja Mutica
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Type of Study: Research | Subject: Microbiology
Received: 2015/12/12 | Accepted: 2016/05/15 | Published: 2016/06/7

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Volume 21, Issue 3 (6-2016) Back to browse issues page