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Indexing & Abstracting
:: Volume 28, Issue 4 (7-2023) ::
__Armaghane Danesh__ 2023, 28(4): 474-490 Back to browse issues page
The Effects of Oak Placenta(jaft) Extract Combined with Cisplatin on Gastric Cancer Cell Line(AGS)
S Andarzi1 , H Sadeghi2 , SM Syyednejad1 , MR Hajari 3
1- Department of biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Department of biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran , Mohamad.hajari@gmail.com
Abstract:   (943 Views)
Background & aim: Oak placenta(Jaft) extract has potent antioxidant, anti-proliferative, anti-cancer activity, and other therapeutic properties. Therefore, the aim of this study is to investigate the effect of Jaft extract and its combination with Cisplatin on gastric cancer adenocarcinoma cell line (AGS).

Methods: In the present experimental study conducted at Shahid Chamran University of Ahvaz in 2022, the AGS cell line was cultured in 1640-RPMI medium and treated with Jaft extract and cisplatin. Cell viability and combination index(CI) were measured using the MTT assay and Compucyn software(respectively). Moreover, the expression of the CDK2 gene as an important gene involved in the cell cycle was evaluated by Real-Time PCR. The collected data were analyzed using Graph Pad Prism 6 software and one-way ANOVA.

Results: The findings of the present study indicated that Jaft extract and cisplatin cause decreased cell viability of AGS (p< 0.0001). The analysis of compusyn data indicated that these two substances together have synergistic properties (CI<1), in addition to simultaneous usage of Jaft extract increases the cytotoxicity of cisplatin drug. In the simultaneous treatment, it was observed that the expression of CDK2 decreased and led to an increase in the anticancer effect of cisplatin (p<0.001).

Conclusion: The present study revealed that placenta extract had anti-proliferative and anti-cancer properties. According to the synergistic effects of these two drugs, Jaft can change the gene expression of CDK2 in the cell cycle and introduce a possible approach for the treatment of gastric cancer with more potency and less dose of administered cisplatin to induce toxicity. The results of the present study indicated that Jaft extract is a proper selection to sensitize cancer cells to cisplatin.

Keywords: Jaft extract, Cisplatin, AGS cell line, Adenocarcinoma, CDK2
Full-Text [PDF 606 kb]   (166 Downloads)    
Type of Study: Research | Subject: Internist
Received: 2023/01/23 | Accepted: 2023/06/20 | Published: 2023/07/10
1. Siegel RL, Miller KD, Jemal A. Cancer statistics 2018. CA: A Cancer Journal for Clinicians 2018; 68(1): 7-30.## [DOI:10.3322/caac.21442] [PMID]
2. Tanida S, Mizoshita T, Ozeki K, Tsukamoto H, Kamiya T, Kataoka H, Sakamuro D, Joh T. Mechanisms of cisplatin-induced apoptosis and of cisplatin sensitivity: potential of BIN1 to act as a potent predictor of cisplatin sensitivity in gastric cancer treatment. International Journal of Surgical Oncology 2012; 2012: 862879. ## [DOI:10.1155/2012/862879] [PMID] []
3. Sedletska Y, Giraud-Panis MJ, Malinge JM. Cisplatin is a DNA-damaging antitumour compound triggering multifactorial biochemical responses in cancer cells: importance of apoptotic pathways. Current Medicinal Chemistry-Anti-Cancer Agents 2005; 5(3): 251-65.## [DOI:10.2174/1568011053765967] [PMID]
4. Lesan V, Ghaffari SH, Salaramoli J, Heidari M, Rostami M, Alimoghaddam K, Ghavamzadeh A. Evaluation of antagonistic effects of metformin with Cisplatin in gastric cancer cells. International Journal of Hematology-Oncology and Stem Cell Research 2014; 8(3): 12.##
5. Najafzadeh N, Abbasi A, Mazani M, Amani M. Effects of all trans retinoic acid combined with cisplatin on survival of gastric cancer cell line(AGS). Scientific Journal of Hamadan University of Medical Sciences Autumn 2013; 20(3): 207-14.##
6. Rabik CA, Dolan ME. Molecular mechanisms of resistance and toxicity associated with platinating agents. Cancer Treatment Reviews 2007; 33(1): 9-23.## [DOI:10.1016/j.ctrv.2006.09.006] [PMID] []
7. Sagar SM, MH DY, Wong RK. Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer. Current Oncology 2006; 13(1): 14-26.## [DOI:10.3747/co.v13i1.77] [PMID] []
8. Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond [J]. Am J Clin Nutr 2005; 81(1 Suppl): 215s-217s.## [DOI:10.1093/ajcn/81.1.215S] [PMID]
9. MY H, WA WA. Cytotoxicity Effect of Quercus infectoria Based Vaginal Cream on Hela Cells and its Preliminary in Vivo Toxicity Evaluation Towards Female Rats. International Medical Journal Malaysia 2018; 17(2): 27-45.## [DOI:10.31436/imjm.v17i2.276]
10. Yarani R, Mansouri K, Mohammadi-Motlagh HR, Mahnam A, Emami Aleagha MS. In vitro inhibition of angiogenesis by hydroalcoholic extract of oak (Quercus infectoria) acorn shell via suppressing VEGF, MMP-2, and MMP-9 secretion. Pharmaceutical Biology 2013; 51(3): 361-8.## [DOI:10.3109/13880209.2012.729147] [PMID]
11. Moradi MT, Karimi A, Alidadi S. In vitro antiproliferative and apoptosis-inducing activities of crude ethyle alcohole extract of Quercus brantii L. acorn and subsequent fractions. Chinese Journal of Natural Medicines 2016; 14(3): 196-202.## [DOI:10.1016/S1875-5364(16)30016-4] [PMID]
12. GraphPad Statistics. Synergism Effect of Drug Combinations in Compusyn Software. Statistical tutorials and software guides. Retrieved January 2022;12: 123-35.##
13. Longchar A, Prasad SB. Biochemical changes associated with ascorbic acid-cisplatin combination therapeutic efficacy and protective effect on cisplatin-induced toxicity in tumor-bearing mice. Toxicology Reports 2015; 2: 489-503.## [DOI:10.1016/j.toxrep.2015.01.017] [PMID] []
14. Ghavami G, Sardari S. Synergistic effect of vitamin C with cisplatin for inhibiting proliferation of gastric cancer cells. Iranian Biomedical Journal 2020; 24(2): 119.## [DOI:10.29252/ibj.24.2.119] [PMID] []
15. Karna P, Gundala SR, Gupta MV, Shamsi SA, Pace RD, Yates C, Narayan S, Aneja R. Polyphenol-rich sweet potato greens extract inhibits proliferation and induces apoptosis in prostate cancer cells in vitro and in vivo. Carcinogenesis 2011; 32(12): 1872-80.## [DOI:10.1093/carcin/bgr215] [PMID] []
16. Sak K. Chemotherapy and dietary phytochemical agents. Chemotherapy Research and Practice 2012; 2012: 45-64.## [DOI:10.1155/2012/282570] [PMID] []
17. Herbst RS. Therapeutic options to target angiogenesis in human malignancies. Expert Opin Emerg Drugs 2006; 11(4): 635-50.## [DOI:10.1517/14728214.11.4.635] [PMID]
18. Soon LK, Hasni E, Law KS, Walliullah SS, Fardi CG, Syed Mohsin SSJ. Ultrastructural findings and elemental analysis of quercus infectoria olive. Ann Microscopy 2007; 7(1): 32-7.##
19. Khennouf S, Benabdallah H, Gharzouli K, Amira S, Ito H, Kim TH, Yoshida T, Gharzouli A. Effect of tannins from Quercus suber and Quercus coccifera leaves on ethanol-induced gastric lesions in mice. J Agric Food Chem 2003; 51: 1469-73.## [DOI:10.1021/jf020808y] [PMID]
20. Umachigi SP, Jayaveera KN, Ashok Kumar CK, Kumar GS, Vrushabendra Swamey BM, Kishore Kumar DV. Studies on wound healing properties of Quercus infectoria. J Pharm Res 2008; 7: 913-9.## [DOI:10.4314/tjpr.v7i1.14677]
21. Aroonrerk N, Kamkaen N. Anti-inflamatory activity of Quercus infectoria, Glycyrrhiza uralensis, Kaemperia galanga and Coptis chinensis, the main components of thai herbal remedies for aphthous ulcer. J Health Res 2009; 23(1): 17-22.##
22. Azizi S, Pirbalouti AG, Amirmohammadi M. Effect of hydro-alcoholic extract of Persian oak (Quercus brantii) in experimentally gastric ulcer. Iranian Journal of Pharmaceutical Research: IJPR 2014; 13(3): 967.##
23. Zingg JM, Azzi A. Nonantioxidant activities of vitamin E. Curr Med Chem 2004; 11(9): 1113-33.## [DOI:10.2174/0929867043365332] [PMID]
24. Gharzouli K, Khennouf S, Amira S, Gharzouli A. Effects of aqueous extracts of Quercus ilex (L.) root bark, Punica granatum (L.) fruit peel and Artemisiaalba Asso leaves on ethanol-induced gastric damage in rats. Phytother Res 1999; 13: 42-5.## https://doi.org/10.1002/(SICI)1099-1573(199902)13:1<42::AID-PTR383>3.0.CO;2-2 [DOI:10.1002/(SICI)1099-1573(199902)13:13.0.CO;2-2]
25. Khennouf S, Gharzouli K, Amira S, Gharzouli A. Effects of Quercus ilex (L.) and Punica granatum.polyphenols against ethanol-induced gastric damage in rats. Pharmazie 1999; 54: 75-6.##
26. Khennouf S, Amira S, Arrar L, Baghiani A. Effect of some phenolic compounds and Quercus tannins on lipid peroxidation. World Appl Sci J 2010; 8: 1144-9.##
27. Amit KT, Madhumita R, Bhattacharya RK. Natural products as inducer of apoptosis: Implication for cancer therapy and prenvention. Curr Sci 2001; 80(11): 1387-96.##
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Andarzi S, Sadeghi H, Syyednejad S, Hajari M. The Effects of Oak Placenta(jaft) Extract Combined with Cisplatin on Gastric Cancer Cell Line(AGS). armaghanj 2023; 28 (4) :474-490
URL: http://armaghanj.yums.ac.ir/article-1-3429-en.html

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Volume 28, Issue 4 (7-2023) Back to browse issues page
ارمغان دانش Armaghane Danesh
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