[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Indexing & Abstracting
:: Volume 29, Issue 1 (3-2024) ::
__Armaghane Danesh__ 2024, 29(1): 17-34 Back to browse issues page
Investigating the Protective Effects of Hydroethanolic Extract of Palm Meristem on Spermatogenesis in Male Wistar Rats Induced with Lead Acetate
Negar Ayazi1 , Naser Mirazi 2, Hesam Parsa1 , Samaneh Safari3
1- Bu-Ali Sina University
2- Bu-Ali Sina University , mirazi205@gmail.com
3- Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:   (645 Views)
Background & aim: Lead is an environmental pollutant and has destructive effects on the functioning of various body systems such as the reproductive system. The aim of the present study was to investigate the protective effect of hydroethanolic extract of palm meristem on the process of spermatogenesis in male Wistar rats induced with lead acetate.
Methods: In the present experimental study conducted in 2023, 35 male Wistar rats with 200-220g were used and divided in five groups: Control (received water and normal food), positive control (received palm meristem extract with a dose of 500 mg/kg, ip), witness (received lead acetate 0.5 gr/ L, in drinking water) and Treatment 1 and 2 (receiver of lead acetate, 0.5 gr/ L, in drinking water and palm meristem extract with a dose of 250 mg/kg and 500 mg/kg, ip, respectively).The experiments were conducted  for 2 weeks. At the end of the experiments, blood and serum were taken from the animals to measure testosterone, LH and FSH hormones, then sperm count and testicular histological samples were evaluated. Collected data were analyzed using Kolmogorov-Smironov statistical tests, one-way analysis of variance within the subject and Tukey's post hoc test.
Results: The results of the present study indicated that lead acetate causes destruction and necrosis in the testicular tissue of rats. It correspondingly caused a significant decrease in the number of sperms and the serum level of testosterone hormone (P<0.001). Palm meristem's extract protected the testicular tissue and increased the process of spermatogenesis in the treatment groups compared to the witness group. Testosterone hormone increased significantly in the treatment groups compared to the witness group (P<0.05).
Conclusion: The hydroethanolic extract of palm meristem had a protective effect on the testicular tissue of rats against the toxic effects of lead acetate.

Keywords: Palm meristem, Spermatogenesis, Testosterone, Lead Acetate, Rat
Full-Text [PDF 836 kb]   (91 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2023/09/21 | Accepted: 2024/01/1 | Published: 2024/01/16
1. Bastampour F, Hosseini S, Shariaty M, Mokhtari M. The Effect of Aqueous Alcoholic extract of parsley Leaf (Petrose linum crispum) on Pituitary-Thyroid hormones in adult male rats following lead acetate Poisoning. Armaghan Danesh.2022;27(2):185-195. [DOI:10.52547/armaghanj.27.2.185]
2. Hatami B, Abdi S, Noori H, Mehrad H. Alterations in liver and thyroid function tests in patients with lead poisoning. Tehran Univ Med J 2022; 80 (1) :48-56.
3. Ranji N,Habibollahi H,Kochakinegad R,Jafarzadeh MA.Eveluation of N-Acetyle Cyctein effects in inhibition of inflammation and tissue damage on the lungs of rats exposed to acute and chronic doses of lead.Study in Medical Sciences.2022;32(11):831-839. [DOI:10.52547/umj.32.11.831]
4. Samarghandian S, Borji A, Afshari R, Delkhosh MB, gholami A.The effect of lead acetate on oxidative stress and antioxidant status in rat bronchoalveolar lavage fluid and lung tissue. Toxicol Mech Methods. 2013;23(6):432-6. [DOI:10.3109/15376516.2013.777136] [PMID]
5. Moradi F, Mahmoudi Khatir N. Investigating the effect of nanoparticles with antioxidant properties on free radicals. Journal Qom Islamic Azad University.2022;12(47):63-86.
6. Zand Vakili F, Zare S, Rahimi K, Riahi M. The effect of Evening Primrose oil on changes in polycystic ovary syndrome induced by estradiol valerate in rat. Armaghan Danesh. 2018; 22(6):714-724.
7. Rajurkar NS, Hande SM. Estimation of Phytochemical Content and Antioxidant Activity of Some Selected Traditional Indian Medicinal Plants. Indian J Pharm Sci. 2011 Mar-Apr; 73(2): 146-151. [DOI:10.4103/0250-474X.91574] [PMID] []
8. Yousefi L,Tehranipour M, Zafar Balanezhad S. Effect of Triticum Sativum hidroalcoholic extract on sex hormones in rats. The Quarterly journal of School of Medicine, Shahid Beheshti University of Medical Sciences.2021;44(4):540-548.
9. Noshad, M, Alizadeh Behbahani B, Rahmati Joneidabad M. Evaluation of antimicrobial activity, antioxidantpotential, total phenolic and flavonoid contents of nettle extract :A laboratory study. Iranian Journal of Food Science and Technology.2022;125(19):147-156. [DOI:10.52547/fsct.19.124.207]
10. Raeeszadeh M, Mortazavi P.The study of the effect of hydroalcoholic extracts of Broccoli on lead induced oxidative stress in kidney of mice. Razi Journal of Medical Sciences.2018;25(9):17-25.
11. Moosavi MA, Shavasi N, Khatibi SA. Investigating the nutritional and therapeutic propertiesof Date in the Quran, hadiths, traditional medicine and modern medicine. Journal of Traditional Medicine of Islam and Iran.2017;8(3).297-308.
12. Abdollahi S, Azarbayjani MA, Peeri M, Rahmati Ahmadabad S.The health related biological effects of Date Palm Pollen (DPP) along with physical activity: A Narrative review. Scientific Rasearch Journal. Sports Science Quarterly.2023;14(46):11-26.
13. Moghadamnia D, Mkhtari M, Alizadeh A. Effect of Phoenix Dactylifera Spathe on seminiferous tubules structure and spermatogenesis in rat. Journal of Gorgan University of Medical Sciences.2016;18(1):39-44.
14. Shariati M, Mokhtari M, Rastegar S.The effect of Aloe Vera extract on the changes of Testosterone and Gonadotropin hormones in adult male rats.Journal of Sabzevar University of Medical Sciences.2009;16(151):12-17.
15. Sangar G, Mirazi N, Hayati Roodbari N. Study of Protective Effects of Palm Meristem's Hydroalcholic Extract on Polycystic Ovary Syndrome in Rats Induced with Estradiol Valerate. armaghanj 2023; 28 (4) :455-473
16. Mirazi N, Shabazi L, Naseri S. Study of Pelargonium graveolensl hydroethanolic leaves extract spermatogenesis in rats induced with lead acetate. The Journal of Urmia University of Medical Sciences.2016;26(11):1001-1009.
17. Roomiani E, Morovvati H, Sadeghinezhad J, Najaf Asaadi S. Evaluation of the effect of lead acetate on rat sperm parameters during the prenatal and lactation periods. Iran J.Vet. Cli. Sci. 2022;15(2):51-58.
18. Khodaei Motlagh M. An overview of the antioxidant effects of silymarin and its role in reducing the harmful effects of some heavy metals, radiation and nicotine on sperm quality. Journal of Cell and Tissue.2023;14(1):1-16. [DOI:10.61186/JCT.14.1.1]
19. Mohebali R, Eidi A, Mortazavi P, Edalatmanesh MA. Effect of Tilia platphy llosl extract on the sex hormones levels and histopathology index in varicocele induced infertile rats.Physiology and January formation.2022;15(1):71-82.
20. Hanafiah Fazhan H, Khor Waiho K, Henrik Glenner H, Moh J, Hassan M, Ikhwanuddin M. Gonadal degeneration and hepatopancreas alteration in orange Mud Crab Scylla olivacea infected with Sacculina beauforti (Crustacea; Rhizocephala; Sacculinidae). Front. Mar. Sci. 2020. https://doi.org/10.3389/fmars.2020.534443 [DOI:10.3389/fmars.2020.534443.]
21. Sadein E, Haghpanah T, Nematollahi Mahani N, Ezzatabadipour M, Dehesh T.. Protective effect of ascorbic acid on sperm parameters and testis structure of the first-generation mouse following mancozeb exposure. Feyz, Journal of Kashan University of Medical Sciences, December, 2021; Vol. 25, No 5, Pages 1155-1167.
22. Ghane Z, Vazini H, Pirestani M. Protective effect of hydroalcoholic extract of Elettaria cardamomum L. Fruits on Serum levels of liver enzymes and morphpological changes in lead induced male rats. J Mazandaran Univ Med Sci.2016;26(142):1-13.
23. Hanachi P, Ghorbnay N, Sadegi A, Aliabadi H, Kiarostami Kh, Hosseini FS. Evaluation of total phenolic and flavonoid compounds of Lavandula angustifolia and Melissa officialis and their antibacterial properties using different Solvents and extraction methods.Mashhad University of Medical Sciences.2022;25(82):38-49.
24. Khezri Motlagh S, Mokhtari M, Shariati M. Evaluation of the protective effects of the hydroalcoholic extract of Securigera Securidacal seeds on altereratons of Serum biochemical parameters and renal indices in Tamsulosin-treat male rats. Department of Kazeron Biology.2023;15(3):121-136.
25. Peivandi S,Khalili Savadkouhi S,Abbasi Z,Zamaniyan M,Gordan N,Moradi S. Effect of Royal Jelly on Sperm parameters and Testosterone levels in infertile men. J Mazandaran Univ Med Sci 2022; 31 (206): 43-52.
26. Shiravi AH,Najafi B,Shajiei H.Investigating the effect of Trigonella foenum-graecum L alcoholic extract on HPG axis and spermatogenesis in NMRI male rat. Animal Biology Research of Damghan Azad University.2010;3(1):51-60.
27. Soleimani Z, Mirazi N.Study of protective effect of Avicennia marina hydroethanolic leaf extract on testes tissue and spermatogenesis in male rat induced with Carbon tetracholoride.Armaghan Danesh. 2015;20(8):677-688.
28. Sheikhzadeh Mamavi E,Karimi A,Pileh Varian AA.The effect of human Gonadotropin and Cinnamon hydroalcoholic extract on testes and seminal vesicels in adult male rats.Journal of Developmental Biology.2021;13(2):59-65.
29. Fadaei S, Asle Rosta M. Anxiolytic and antidepressant effects of cinnamon (Cinnamomum verum) extract in rats receiving lead acetate.SJKV.2018;22(6):31-39.
30. Morovvati H,Moradi HR,Adibmoradi M,Sheybani MT,Salar Amoli J.Wheat sprout effects on histological and histometrical structure and sperm parameters in testis of rat exposed to lead.Journal of Veterinary Research.2017;72(1):87-101.
31. Abuelgassim A , Eltayeb M, Ataya F. Palm date (Phoenix dactylifera) seeds: A rich source of antioxidant and antibacterial activities. Czech J. Food Sci., 2020, 38(3):171-178 | DOI: 10.17221/269/2019-CJFS [DOI:10.17221/269/2019-CJFS]
32. Karimi Jashni H, Dokhanchy F, Hooshmand F. Effect of hydroalcoholic palm meristem extract on the serum hormone Testosterone,Estradiol and Gonadotropin male rats. Journal of Pars Medical Sciences.2022;8(3):1-6. [DOI:10.29252/jmj.8.3.1]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ayazi N, Mirazi N, Parsa H, Safari S. Investigating the Protective Effects of Hydroethanolic Extract of Palm Meristem on Spermatogenesis in Male Wistar Rats Induced with Lead Acetate. armaghanj 2024; 29 (1) :17-34
URL: http://armaghanj.yums.ac.ir/article-1-3522-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 1 (3-2024) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.05 seconds with 38 queries by YEKTAWEB 4657